Basic Information

• CAS Number: 122111-03-9

• Product Name: Gemcitabine Hydrochloride

• Molecular Formula: C9H11F2N3O4•HCl

• Molecular Weight: 299.66

• Appearance: White powder

• Purity: >99%

• Solubility: Soluble in water (50 mM or ≥10 mg/mL)

1. Physical Properties:

• Melting Point: >250°C dec. (Note: The melting point of gemcitabine base is 168.64°C, but the hydrochloride salt may have a different melting point.)

• Specific Rotation: D +48°; 365 +257.9° (c = 1.0 in deuterated water)

• Stability: It remains stable for 1 year from the date of purchase. Solutions in DMSO or distilled water can be stored at -20°C for up to 1 month.

2. Mechanism of Action:

• Gemcitabine is a deoxycytidine analog that is activated within cells by nucleoside kinases to form gemcitabine diphosphate (dFdCDP) and gemcitabine triphosphate (dFdCTP).

• dFdCDP inhibits ribonucleotide reductase, thereby inhibiting DNA synthesis.

• dFdCTP is incorporated into replicating DNA chains, terminating chain elongation and inducing cell apoptosis.