I. Basic Information

• Product Name: Doxycycline hydrochloride

• CAS Number: 10592-13-9

• Molecular Formula: C22H25ClN2O8

• Molecular Weight: 480.90 (or 480.896)

II. Physical Properties

• Appearance: Light blue or yellow crystalline powder, also described as a powdered form in some sources.

• Solubility: Easily soluble in DMSO, water, and methanol; slightly soluble in ethanol and acetone.

• Purity: Typically ≥98%

• Density: Approximately 1.63 g/cm³

• Boiling Point: 762.6ºC at 760 mmHg (or 773.2ºC at 760 mmHg, with slight variations in data from different sources)

• Flash Point: 415ºC (or 421.4ºC, with slight variations in data from different sources)

• Melting Point: 195-201°C

III. Chemical Properties and Biological Activity

• Chemical Properties: Odorless with a bitter taste, and hygroscopic.

• Biological Activity:

• Orally effective broad-spectrum metalloproteinase (MMP) inhibitor.

• Has antibacterial activity against both Gram-positive cocci and Gram-negative bacilli, with a broader antibacterial spectrum and approximately 10 times stronger antibacterial activity than tetracycline. It is also effective against tetracycline-resistant bacteria.

• Demonstrates anticancer cell proliferation activity. In glioma cell lines, high concentrations of doxycycline hydrochloride can inhibit cell growth.

IV. Uses and Applications

• Medical Uses: Used to treat various infections caused by sensitive bacteria, such as rickettsial diseases, Mycoplasma infections, and Chlamydia infections.

• Research Uses: In molecular biology research, it is often used as an inducer to induce gene expression. In cells or model animals with a tetracycline (Tetracycline)-inducible expression (Tet-ON/Tet-OFF) system constructed, the expression of the target gene can be precisely controlled ON and OFF by adding or removing doxycycline.